An improved method for concentrating rotavirus from water samples

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An improved method for concentrating rotavirus from water samples.

A modified adsorption-elution method for the concentration of seeded rotavirus from water samples was used to determine various factors which affected the virus recovery. An enzyme-linked immunosorbent assay was used to detect the rotavirus antigen after concentration. Of the various eluents compared, 0.05M glycine, pH 11.5 gave the highest rotavirus antigen recovery using negatively charged me...

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ژورنال

عنوان ژورنال: Memórias do Instituto Oswaldo Cruz

سال: 2001

ISSN: 0074-0276

DOI: 10.1590/s0074-02762001000600013